بسم الله الرحمن الرحيم
These are some types of blood coagulants.
1_Potassium Oxalates
This
a counter to the deposition of ions of calcium and thus prevents blood
clots and preferably used for ease of melting, and we need usually to 10
to 20 mg of oxalate a potassium to prevent the clot 10 ml of the blood
and 2 mg per one ml of the blood and used This
converter is usually a concentration of 30% and standardized to pH PH =
7.4 by adding potassium hydroxide solution or a solution of oxalic acid
It is worth mentioning that 0.1 ml of a solution of potassium oxalate
reddish enough to prevent clotting 10 ml of blood.
2 - Sodium fluoride
Is
usually used as a preservative in order to estimate the glucose in the
blood but it is used as an anti-clotting (weak), and when used as a
preservative as well as the presence of anti-clotting, such as oxalate
of potassium, it would be effective concentration of about 2 mg / 1 ml
of blood and starts its effect by inhibiting the enzymatic
common in the process of Glycolysis, which leads to a lack of focus,
and prepare pipe container for this to the mix dissolving 4 g of sodium
chloride with 12 g of oxalate of potassium in 200 ml of water, put one
drop in each tube for 1 ml of blood and dried tubes at a temperature less than 100 m.
As
a general rule? Since fluoride should not be used when collecting
samples for estimates of enzyme or when used in the test reagent Reagent
(length enzyme), such as urease Urease method for estimating urea.
3 - Ethylene diamine four-acetic acid
Ethylene Diamine Tetra Acetic Acid (EDTA)
Prefer
to use this counter in tests of Hematology Hematology in particular,
where he works to maintain the cellular components from damage and is
commonly used in salt disodium or binary potassium concentration of
approximately 1 to 2 mg / ml of blood and attributed the effectiveness
of this Salt as an anticoagulant to its ability to link with calcium in whole blood and isolated play a role in the process of coagulation.